The Samjhota Express has resumed. The train departed from Lahore for India with 150 passengers on Monday.
The train runs between the two countries on Mondays and Thursdays.
service was halted due to escalating Pakistan-India tensions.
Indian passengers were stranded at the Lahore railway station for the past four days.
Tensions escalated dramatically between Pakistan and India on Feb.14 when a suicide bomber rammed an explosives-laden car into an Indian military convoy.
At least 44 soldiers were killed in the attack.
Indian fighter jets in response attacked Balakot in Pakistan.
Pakistani fighter jets attacked Indian held Kashmir downing two Indian planes that caused rise in tensions between the two countries.